Journal
FOOD CHEMISTRY
Volume 351, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.129286
Keywords
Zein; Zearalenone; Maize; Hidden zearalenone; Interaction; Mechanism
Funding
- National Natural Science Foundation of China [32072137]
- Fundamental Research Funds for the Central Universities [XDJK2020B044]
- Venture & Innovation Support Program for Chongqing Overseas Returnees [cx2018032]
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This study investigated the formation of ZEN-zein complex using ultrafiltration, multi-spectroscopic, and molecular modeling techniques. The results showed that ZEN can interact with zein to form a complex driven by hydrophobic force and hydrogen bonds. The conformational changes induced by binding with ZEN were revealed by Fourier transform infrared spectroscopy and circular dichroism.
Hidden mycotoxins have been reported to be ?protected? by macromolecular substances to escape routine determination, but release to free mycotoxins under gastrointestinal conditions. Nowadays, the hidden zear-alenone (ZEN) that binding with macromolecular zein has been found in maize. However, the binding mecha-nism of ZEN with zein in maize has not been clarified. In this study, the formation of ZEN-zein complex was investigated applying ultrafiltration, multi-spectroscopic and molecular modeling techniques. The steady-state and transient fluorescence analysis suggested the ZEN could interact with zein to form the complex driven by hydrophobic force and hydrogen bonds, which is in accordance with the molecular modeling studies. The conformational changes of zein induced by binding with ZEN were revealed by Fourier transform infrared spectroscopy (FTIR) and circular dichroism (CD). Elucidating the binding mechanism between zein and ZEN could help the development of detecting hidden ZEN and guarantee the safety of maize products.
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