Journal
FOOD CHEMISTRY
Volume 347, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.129069
Keywords
Alicyclobacillus acidoterrestris; Monoclonal antibody; Carbon dots; Fluorescence resonance energy transfer; Fluorescence immunoassay
Funding
- National Natural Science Foundation of China [31671866]
Ask authors/readers for more resources
A novel fluorescence immunoassay using monoclonal antibody against A. acidoterrestris and carbon dots as the signal response unit was developed, showing enhanced accuracy and sensitivity with a low limit of detection. The method can detect contaminated apple juice after 24-hour enrichment, reducing the adverse effect on fruit juice quality.
The growth and metabolism of Alicyclobacillus acidoterrestris can lead to the spoilage of commercial fruit juice. Existing methods have some drawbacks such as complex sample pretreatment, skilled technician requirement, reduced sensitivity and specificity. Herein, a novel fluorescence immunoassay was developed using a monoclonal antibody (mAb) against A. acidoterrestris as the sensing element and carbon dots (CDs) as the signal response unit. The CDs can be quenched via fluorescence resonance energy transfer (FRET) by the oxidization product of pphenylenediamine (PPD), a chromogenic substrate of horseradish peroxidase (HRP). This approach showed enhanced accuracy and sensitivity with relatively low limit of detection (LOD) of 6.16 x 10(2) CFU mL(-1). Moreover, apple juice contaminated with 1 CFU mL(-1) of A. acidoterrestris can be identified after 24 h enrichment. This fluorescence immunoassay could serve as a powerful tool for laboratory identification and on-site inspection of A. acidoterrestris, reducing the adverse effect on the quality of fruit juice.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available