Journal
FOOD CHEMISTRY
Volume 357, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.129766
Keywords
Black phosphorus-Platinum nanocomposites; Nanozyme sensing probes; One step colorimetric; Photothermal; Immunosensing assay; Quantitative detection
Funding
- 68th China Postdoctoral Science Foundation [2020M680870]
- National Key R&D Program of China [2016YFD0401202]
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Nanozymes constructed using two-dimensional black phosphorus nanosheets show high catalytic performance in immunosensing, enabling sensitive detection of enrofloxacin. Additionally, a photothermal immunosensing assay based on the photothermal performance of oxTMB achieved similar detection results.
The two-dimensional black phosphorus nanosheets (BPNSs) provide strong support for the construction of nanozymes with high catalytic performance due to the sheet structure and high electronic activity. A peroxidaselike BP-Pt nanocomposites was successfully synthesized using the instability of BPNS, a non-enzymatic immunosensing assay (NISA) was established with BP-Pt as immunosensing probe. Take the antibiotic enrofloxacin (ENR) as the target, NISA realized the highly sensitive ENR detection with detection limit (IC15) of 0.005 mu g/L. In addition, based on the good photothermal performance of oxTMB at 808 nm, a photothermal immunosensing assay (PT-NISA) was established, and ENR detection results was similar to NISA were obtained. In the analysis of the samples, the same detection results as the commercially available enzyme-linked immunoassay kit were obtained. These NISA and PT-NISA provide a more rapid and promising strategy for detecting food contaminants, and was expected to be used to detect other highly sensitive biological macromolecules.
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