Journal
FOOD CHEMISTRY
Volume 346, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2020.128894
Keywords
Acetylcholinesterase; Tribolium castaneum (Red flour beetle); Pencil graphite electrode (PGE); Graphitic carbon nitride; Tungsten oxide; Phosmet; Docking
Funding
- COMSATS University
- Pakistan Science Foundation [PSFMSRTII/AGR/P-COMSATS-LHR (09)/2019]
Ask authors/readers for more resources
This study successfully developed a biosensor using WO3/g-C3N4 nanocomposite-modified electrode for detecting the organophosphate insecticide Phosmet with good analytical performance, especially in wheat samples.
This study was undertaken to assess the potential of Tribolium castaneum (Red flour beetle) acetylcholinesterase (Tc-AChE) based electrochemical biosensor integrating WO3/g-C3N4 nanocomposite modified Pencil graphite electrode to detect an organophosphate insecticide, Phosmet. The WO3/g-C3N4 nanocomposite provides a non-toxic, biocompatible surface for binding the enzyme on the electrode surface, attributed to its large surface area, high conductivity, and low ohmic resistance. The proposed biosensor shows a very good analytical performance with LOD 3.6 nM for Phosmet and effectively determined Phosmet in wheat with a 99% recovery rate. Furthermore, molecular docking deciphers the binding interactions of Phosmet with Tc-AChE using a modified AutoDock LGA algorithm and an AMBER03 force field in YASARA. The kinetic parameters strongly suggest the high potency of inhibitor with the enzyme. This study presents an adaptable, rapid, and straightforward approach that opens ways towards real progress in developing commercial biosensors for pesticide detection.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available