Journal
FOOD CHEMISTRY
Volume 347, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.129024
Keywords
Bio-barcode; Biochip silver staining; Immunoassay; Triazophos; Gold nanoparticles
Funding
- National Key Research Program of China [2016YFD0401101]
- NIEHS Superfund Research Program [P42 ES04699]
- Ningbo Innovation Project for AgroProducts Quality and Safety [2019CXGC007]
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A novel visual method for detecting triazophos using competitive bio-barcode immunoassay was established, with a detection range between 0.05 and 10 ng/mL. The method showed high percent recovery and stability, offering a new insight for rapid detection of small molecule pesticides in various matrices.
Herein, a novel visual method for detecting triazophos based on competitive bio-barcode immunoassay was described. The competitive immunoassay was established by gold nanoparticles (AuNPs), magnetic microparticle (MMPs) and triazophos, combined with biochip hybridization system to detect the residual of triazophos in water and apple. Because AuNPs carried many bio-barcodes, which hybridized with labeled DNA on the biochip, catalyzed signal amplification using silver staining was detected by grayscale values as well as the naked eye. Notably, the grayscale values decreases with increasing the concentrations of triazophos, and the color change weakened gradually. The detection range was in between 0.05 and 10 ng/mL and the minimum detection limit was set at 0.04 ng/mL. Percent recovery calculated from spiked water and apple samples ranged between 55.4 and 107.8% with relative standard deviations (RSDs) of 12.4-24.9%. It has therefore been shown that this protocol provides a new insight for rapid detection of small molecule pesticides in various matrices.
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