Simultaneous quantification of 12 veterinary drug residues in fishery products using liquid chromatography-tandem mass spectrometry

Herein, an analytical method was developed for simultaneous determination of 12 anthelmintics (closantel, niclosamide, nitroxynil, rafoxanide, cymiazole, fluazuron, levamisole, morantel, praziquantel, pyrantel, thiophanate, and trichlorfon) in fishery products (eel, flatfish, and shrimp) using liquid-liquid extraction coupled with liquid chromatography-tandem mass spectrometry. A reversed-phase analytical column was then used to separate the analytes from various matrices. Linear matrix-matched calibration curves were generated with coefficients of determination >= 0.9935. Recovery rates at three spiking levels (5, 10, and 20 mu g/kg) ranged between 61.58% and 119.37% with relative standard deviations <= 19.05%. Limits of detection were in the range of 0.3-1.6 mu g/kg, whereas limits of quantification ranged between 1.0 and 5.0 mu g/kg. The matrix effect was moderate with values ranging from-99.47% to 51.98%. Matrices procured from large markets tested negative for the 12 anthelmintics. The developed method proved amenable to real sample testing and can be used for simultaneous determination of target analytes in aquatic products.

Automated summary

An analytical method for the simultaneous determination of 12 anthelmintics in fishery products was developed and proved suitable for real sample testing. This method utilized liquid-liquid extraction coupled with liquid chromatography-tandem mass spectrometry and exhibited moderate matrix effects.