Sensitive surface-enhanced Raman spectroscopy (SERS) determination of nitrofurazone by β-cyclodextrin-protected AuNPs/γ-Al2O3 nanoparticles

A novel surface-enhanced Raman spectroscopy (SERS) method for the determination of nitrofurazone was developed using AuNPs/gamma-Al2O3 nanoparticles protected by beta-cyclodextrin (beta-CD) as substrate prepared in our lab. The optimum experimental conditions were obtained from single factor procedure and response surface modeling. A linear relationship (I-SERS = 508.96c + 31987.87, c: nmol L-1, R-2 = 0.996) between SERS intensity and the concentration of nitrofurazone in the range of 3.3 - 667.0 nmol L-1 was established, the limit of detection (LOD) was found at nmol L-1 level (0.37 nmol L-1 by 3S(0)/S). The selectivity for the method was studied by the influences of foreign substances on the determination. The recoveries and RSD (n = 5) for the six meat samples were 95.1 % - 104.5% and 2.4 % - 4.8% respectively, which suggesting that the new SERS method was successfully to detecting nitrofurazone.

Automated summary

A novel SERS method was developed for the determination of nitrofurazone, showing a linear relationship between SERS intensity and nitrofurazone concentration. The method demonstrated good selectivity and accuracy when tested on six meat samples, indicating its effectiveness in detecting nitrofurazone.