The role of cell wall polysaccharides disassembly in Lasiodiplodia theobromae-induced disease occurrence and softening of fresh longan fruit

Cell wall polysaccharides in fruits act a pivotal role in their resistance to fungal invasion. Lasiodiplodia theobromae (Pat.) Griff. & Maubl. is a primary pathogenic fungus causing the spoilage of fresh longan fruit. In this study, the influences of L. theobromae inoculation on the disassembly of cell wall polysaccharides in pericarp of fresh longans and its association with L. theobromae-induced disease and softening development were investigated. In contrast to the control, samples with L. theobromae infection showed more severe disease development, lower firmness, lower amounts of cell wall materials, covalent-soluble pectin, ionic-soluble pectin, cellulose and hemicellulose, whereas higher value of water-soluble pectin, higher activities of cell wall polysaccharide-disassembling enzymes (cellulase, beta-galactosidase, polygalacturonase and pectinesterase). These findings revealed that cell wall polysaccharides disassembly induced by enzymatic manipulation was an essential pathway for L. theobromae to infect harvested longans, and thus led to the disease occurrence and fruit softening.

Automated summary

The disassembly of cell wall polysaccharides in fresh longans induced by enzymatic manipulation is essential for the invasion of Lasiodiplodia theobromae, leading to disease occurrence and fruit softening. Infection by L. theobromae resulted in more severe disease development, lower firmness, decreased cell wall materials and pectin content, and increased activities of cell wall polysaccharide-disassembling enzymes.