Journal
FOOD CHEMISTRY
Volume 357, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2021.129739
Keywords
Food safety; Gutter oil; Capsaicin; Lateral flow strip; Time-resolved fluorescent microspheres
Funding
- Anhui Key RD Project [201904d07020016]
- Ministry of Science and Technology of China [2018YFC1603606, 2017YFF0208600]
- NSFC [21804028]
- China Agriculture Research System-48 (CARS-48)
- Anhui Provincial Natural Science Foundation [1908085QC121]
- China Postdoctoral Science Foundation [225351]
- Fundamental Research Fund for Central University [JZ2019HGTB0068, PA2017GDQT0018, EWPL201905]
- National College Students Innovation and Entrepreneurship Training Program [201910359095]
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The study utilized time-resolved fluorescent lateral flow strip technology, with capsaicin as the marker for gutter oil, successfully applied for rapid screening of gutter oil in edible oil. This method is simple and quick, making it an effective tool for quality monitoring of edible oil.
Gutter oil is strictly prohibited from being reprocessed back to the catering and food industry. Extensive attention has been paid to rapid screening of gutter oil to guarantee the safety of edible oil. Capsaicin, a special component of condiments, has been adopted as the marker of gutter oil. The time-resolved fluorescent microspheres are utilized for labeling of antibody to capsaicin, which are further applied for the construction of fluorescent lateralflow-strip (LFS). By simple extraction of capsaicin with ethanol (or liquor) from the edible oil, the capsaicin can be rapid determined with the fluorescent LFS in less than 10 min. As low as 20 ng/mL capsaicin can be visually judged and 2.3 ng/mL is achieved as the detection limit by ImageJ analysis. The illegal gutter oil is also well screened with this time-resolved LFS. This method can be a useful candidate for routine quality monitoring of edible oil and a powerful tool for self-inspection at home.
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