Journal
FOOD ADDITIVES AND CONTAMINANTS PART A-CHEMISTRY ANALYSIS CONTROL EXPOSURE & RISK ASSESSMENT
Volume 38, Issue 9, Pages 1506-1519Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/19440049.2021.1933206
Keywords
Garlic; bioaccumulation; enriched crop; growth stages; selenium speciation
Funding
- CAPES [88887.115406/2015]
- Fondo para la Investigacion Cientifica y Tecnologica [PICT2016-2506]
- Fundacao de Amparo a Pesquisa do Estado de Sao Paulo [2018/25207-0]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico [401170/2016-0]
- National Institute of Science and Technology for Bioanalytics [MCT465389/2014-7]
- Universidad Nacional de Cuyo [06/M129]
Ask authors/readers for more resources
This study found that garlic has a significant ability to accumulate and metabolize selenium, especially in red clones. Selenium-amino acids were mainly found in garlic bulbs, indicating potential biological properties.
Selenium (Se) is an essential micronutrient for humans. Garlic (Allium sativum L.) metabolises Se into important Se-amino acids like Se-methylselenocysteine (Se-MetSeCys), precursor of methylselenol, an active species for cancer prevention. Therefore, the Se accumulation and speciation in garlic were studied to evaluate their relations with growth stages and types of plant clones. Four garlic clones (Nieve INTA, Union FCA, Gostoso INTA and Rubi INTA) were fortified with a Se solution (169 g Se L-1). The association of Se to different molecular weight fractions was evaluated by size-exclusion chromatography coupled to inductively coupled plasma mass spectrometry (SEC-ICP-MS) detection. Also, anion exchange chromatography (AEC-ICP-MS) was used for the determination of Se-amino acids, while their identification was performed by ESI-MS/MS. The Se was incorporated into high (7-5 kDa) and low (2-4 kDa) molecular weight fractions. The presence of Se-MetSeCys was observed mostly. Se-MetSeCys increased in bulbs to a maximum value but increased, then decreased, in leaves and roots. The Se-organic species were mostly found in bulbs in the last growth stage. Garlic showed a significant ability to accumulate and metabolise Se, specially, the red clones (Gostoso INTA and Rubi INTA). Also, this work suggests that this plant may become an attractive source of Se-amino acids with important biological properties.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available