Journal
FISH & SHELLFISH IMMUNOLOGY
Volume 113, Issue -, Pages 20-23Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.fsi.2021.03.006
Keywords
Smallmouth bass; Micropterus dolomieu; Largemouth bass; Micropterus salmoides; Bluegill; Lepomis macrochirus; Monoclonal antibody; Total plasma immunoglobulin; ELISA
Funding
- Pennsylvania Department of Environmental Protection (PA DEP) [4000020352]
- U.S. Geological Survey, Ecosystems Mission Area, Priority Ecosystems Program
- U.S. Geological Survey, Environmental Health, Contaminants Biology Program
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A murine monoclonal antibody specific for IgM heavy chain in smallmouth bass was produced and shown to also recognize Ig heavy chains in largemouth bass and bluegill. When used in inhibition kinetic assay, the mAb was found to be effective in analyzing total plasma IgM in smallmouth bass.
A murine monoclonal antibody (mAb, IgG2a) was produced for the detection of smallmouth bass (Micropterus dolomieu) immunoglobulin (IgM). The antibody is specific for IgM heavy chain and was shown to also recognize the Ig heavy chain of largemouth bass (Micropterus salmoides) and bluegill (Lepomis macrochirus) using Western Blot analysis of plasma from 9 teleost taxa. When applied to the analysis of smallmouth bass total plasma IgM using ELISA, the mAb was found to be effective when used in an inhibition kinetic assay.
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