Journal
EXPERIMENTAL EYE RESEARCH
Volume 210, Issue -, Pages -Publisher
ACADEMIC PRESS LTD- ELSEVIER SCIENCE LTD
DOI: 10.1016/j.exer.2021.108643
Keywords
Luteolin; Choroidal melanoma; Proliferation; Adhesion; Migration and invasion; MMPs; PI3K/Akt
Categories
Funding
- Biology mechanism research on prevention and control of low power semiconductor laser exposure of hydroxyapatite prosthesis pedestal [20181BBG70007]
- Innova-tion Fund Designated for Graduate Students of Jiangxi Province [YC2019-S040]
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Luteolin has inhibitory effects on choroidal melanoma cells and acts through the PI3K/Akt signaling pathway. This study provides a new theoretical basis for the potential use of luteolin as a therapeutic medication for choroidal melanoma.
Choroidal melanoma is a devastating disease that causes visual loss and a high mortality rate due to metastasis. Luteolin, a potential anticancer compound, is widely found in natural plants. The aim of this study was to evaluate the antiproliferative, antiadhesive, antimigratory and anti-invasive effects of luteolin on choroidal melanoma cells in vitro and to explore its potential mechanism. Cell counting kit-8 (CCK-8) assays, 5-ethynyl-2'-deoxyuridine (EdU) assays, Cell adhesion, migration, and invasion assays were performed to examine the inhibitory effects of luteolin on cell cell viability, proliferation, adhesion, migration and invasion capacities, respectively. Considering the correlation between Matrix metalloenzymes and tumor metastasis, Enzyme-linked immunosorbent assays (ELISAs) were used to assess matrix metalloproteases MMP-2 and MMP-9 secretion. Western blotting was performed to detect p-PI3K P85, Akt, and p-Akt protein expression. The cytoskeletal proteins vimentin were observed with cell immunofluorescence staining. Luteolin can inhibit OCM-1 cell proliferation, migration, invasion and adhesion and C918 cell proliferation, migration, and invasion through the PI3K/Akt signaling pathway. Therefore, Luteolin may have potential as a therapeutic medication for Choroidal melanoma.
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