The functional role of decorin in corneal neovascularization in vivo

Our earlier decorin (Dcn) gene overexpression studies found that the targeted Dcn gene transfer into the cornea inhibited corneal angiogenesis in vivo using a rabbit model. In this study, we tested the hypothesis that antiangiogenic effects of decorin in the cornea are mediated by alterations in a normal physiologic balance of pro- and anti-angiogenic factors using decorin deficient (Dcn -/- ) and wild type (Dcn +/+) mice. Corneal neovascularization (CNV) in Dcn - /- and Dcn +/+ mice was produced with a standard chemical injury technique. The clinical progression of CNV in mice was monitored with stereo- and slit-lamp microscopes, and histopathological hematoxylin and eosin (H&E) staining. Protein and mRNA expression of pro- and anti-angiogenic factors in the cornea were evaluated using immunofluorescence and quantitative real-time PCR, respectively. Slit-lamp clinical eye examinations revealed significantly more CNV in Dcn-/- mice than the Dcn +/+ mice post-injury (p < 0.05) and AAV5-Dcn gene therapy significantly reduced CNV in Dcn- /- mice compered to no AAV5-Dcn gene therapy controls (p < 0.001). H&E-stained corneal sections exhibited morphology with several neovessels in injured corneas of the Dcn - /- mice than the Dcn +/+ mice. Immunofluorescence of corneal sections displayed significantly higher expression of alpha-smooth muscle actin (alpha-SMA) and endoglin proteins in Dcn-/- mice than Dcn +/+ mice (p < 0.05). Quantitative real-time PCR found significantly increased mRNA levels of pro-angiogenic factors endoglin (2.53-fold; p < 0.05), Vegf (2.47-fold; p < 0.05), and Pecam (2.14-fold; p < 0.05) and anti-angiogenic factor Vegfr2 (1.56-fold; p < 0.05) in the normal cornea of the Dcn -/- mice than the Dcn +/+ mice. Furthermore, neovascularized Dcn - /- mice corneas showed greater increase in mRNA expression of pro-angiogenic factors endoglin (4.58-fold; p < 0.0001), Vegf (4.16-fold; p < 0.0001), and Pdgf (2.15-fold; p < 0.0001) and reduced expression of anti-angiogenic factors Ang2 (0.12-fold; p < 0.05), Timp1 (0.22-fold; p 0.05), and Vegfr2 (0.67-fold; p 0.05) compared to neovascularized Dcn +/+ mice corneas. These gene deficience studies carried with transgenic Dcn -/- mice revealed decorin's role in influencing a physiologic balance between pro-and antiangiogenic factors in the normal and injured cornea. We infer that the functional deletion of Dcn promotes irregular corneal repair and aggravates CNV.

Automated summary

The gene deficiency studies conducted with transgenic Dcn -/- mice revealed the role of decorin in influencing the physiologic balance between pro- and antiangiogenic factors in the normal and injured cornea. These findings suggest that the functional deletion of Dcn promotes irregular corneal repair and exacerbates corneal neovascularization.