Journal
EXPERIMENTAL CELL RESEARCH
Volume 403, Issue 2, Pages -Publisher
ELSEVIER INC
DOI: 10.1016/j.yexcr.2021.112598
Keywords
Cyclic mechanical stretch; Human periodontal ligament stem cells; Osteogenic differentiation; Reactive oxygen species; Nuclear factor erythroid-2-related factor-2; Orthodontic tooth movement
Categories
Funding
- National Natural Science Foundation of China [81571010]
- Clinical Research Center of Shandong University [2020SDUCRCA005]
- Graduate education outstanding achievement training program of Shandong University [ZY20190004]
Ask authors/readers for more resources
This study demonstrated the significant role of Nrf2 in PDLSCs under cyclic mechanical stretch, with Nrf2 activation promoting osteogenic differentiation and improving alveolar bone microstructure. Knocking down Nrf2 increased ROS formation and suppressed osteogenic differentiation. T-BHQ, a Nrf2 activator, was shown to enhance osteogenic differentiation both in vitro and in vivo, suggesting a promising option for alveolar bone remodeling during orthodontic tooth movement.
During orthodontic treatment, mechanical stretch serves a crucial function in osteogenic differentiation of periodontal ligament stem cells (PDLSCs). Up-regulated reactive oxygen species (ROS) level is a result of cyclic mechanical stretch in many cell types. Nuclear factor erythroid-2-related factor-2 (Nrf2) is a master regulator in various antioxidants expression. However, it is not known whether cyclic mechanical stretch could induce the ROS generation in PDLSCs and whether Nrf2 participated in this process. The present study was aimed to investigate the role of Nrf2 in PDLSCs under cyclic mechanical stretch. Our results showed that cyclic mechanical stretch increased ROS level and the nuclear accumulation of Nrf2 during osteoblast differentiation. Knocking down Nrf2 by siRNA transfection increased ROS formation and suppressed osteogenic differentiation in PDLSCs. T-BHQ, a Nrf2 activator, promoted the osteogenic differentiation in PDLSCs under cyclic mechanical stretch, and improved the microstructure of alveolar bone during orthodontic tooth movement in rats by employing micro-CT system. Taken together, Nrf2 activation was involved in osteogenic differentiation under cyclic mechanical stretch in PDLSCs. T-BHQ could promote the osteogenic differentiation in vitro and in vivo, suggesting a promising option for the remodeling of the alveolar bone during orthodontic tooth movement.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available