Journal
EUROPEAN JOURNAL OF PLANT PATHOLOGY
Volume 161, Issue 3, Pages 543-552Publisher
SPRINGER
DOI: 10.1007/s10658-021-02340-6
Keywords
Ri-plasmid; Hairy root; Root mat; Detection; Phylogeny
Categories
Funding
- New bacterial diseases of horticultural plants in Poland - Ministry of Science and Higher Education [2.1.5]
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In April 2019, hydroponically cultivated cucumber plants with symptoms of crazy root disease were found in two commercial production greenhouses in Poland. Isolated bacteria from the roots were identified as potential new species causing the disease, requiring further phylogenetic studies for confirmation.
In April 2019, hydroponically cultivated cucumber plants with characteristic symptoms of crazy root disease were found in two different commercial production cucumber greenhouses in Poland. Due to excessive and inappropriate root growth, this disease led to a reduction in yield and deterioration of the general conditions of infected plants. Bacteria isolated from the roots were subjected to a morphological evaluation, as well as molecular, biochemical and pathogenicity tests. To identify the bacteria causing the disease, Agrobacterium-like colonies were subjected to PCR with primers complementary to the pathogenicity-related genes located on the crazy root-inducing plasmid (Ri-plasmid): the virD2A + virD2E primers complementary to the virD2 gene and the rolBF + rolBR primers complementary to the rolB gene. The pathogenicity of the isolated strains was studied in sunflowers and cucumbers. Twelve strains positive for the Ri plasmid, as determined by PCR, and pathogenic to sunflowers were identified based on sequence analysis of the 16S rRNA and recA genes. One strain was classified as belonging to the genus Pararhizobium, three to Rhizobium, and eight to Agrobacterium biovar 1, with the highest similarity to genomospecies G3. The results of the analyses suggest that these strains may belong to a new, thus far, undescribed species. To confirm this hypothesis, further phylogenetic studies are required.
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