Journal
EUROPEAN JOURNAL OF IMMUNOLOGY
Volume 51, Issue 9, Pages 2306-2316Publisher
WILEY
DOI: 10.1002/eji.202049070
Keywords
breast cancer; CD8 T cells; TCR; TCR repertoire; tumor immunology
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Funding
- JSPS KAKENHI [JP16H06499, JP18K07260]
- Project for Cancer Research and Therapeutic Evolution from AMED [21cm0106351h0003, 21cm0106373h0002, 21cm0106371h0002]
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Tumor-infiltrating lymphocytes (TILs) are a valuable source for obtaining tumor-reactive T cell receptors (TCRs). A new evaluation system called c-FIT was developed in this study, which allowed for efficient analysis of up to 90 TCRs for their reactivity to tumor cells within a month. The system revealed interesting findings, including the identification of both HLA I-restricted and non-HLA-restricted TCRs reactive to tumor cells in CD8(+) TILs from breast cancer patients.
Tumor-infiltrating lymphocytes (TILs) are a potent source for obtaining tumor-reactive T cell receptors (TCRs). Although comprehensive methods to analyze the TCR repertoire in TILs have been reported, the evaluation system for TCR-reactivity to endogenously expressed antigen in tumor cells remains laborious and time consuming. Consequently, very limited numbers of TCRs in TILs have been analyzed for their reactivity to tumor cells. In this study, we developed an efficient evaluation system for TCR function designated c-FIT (comprehensive functional investigation of TCRs) to analyze TCR reactivity. The c-FIT system enabled us to analyze up to 90 TCRs for their reactivity to tumor cells by a single assay within a month. Using c-FIT, we analyzed 70 TCRs of CD8(+) TILs derived from two breast cancer patients and obtained 23 TCRs that reacted to tumor cells. Surprisingly, although two TCRs were HLA class I-restricted, the remaining 21 TCRs were non-HLA-restricted. Thus, c-FIT can be applied for monitoring multiple conventional and unconventional antigen-specific killer T cells in TILs, leading to the development of new designs for more effective T-cell-based immunotherapies.
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