Journal
EUROPEAN JOURNAL OF HISTOCHEMISTRY
Volume 65, Issue -, Pages -Publisher
PAGEPRESS PUBL
DOI: 10.4081/ejh.2021.3241
Keywords
Neurons; SUMO; synapse; hippocampus; cortex
Categories
Funding
- Brightfocus Grant [A2019296F]
- Fondo di Beneficenza Gruppo Intesa Sanpaolo
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Recent study focused on investigating the co-localization of PIAS1 and PIAS3 with synaptic markers in hippocampal and cortical murine neurons, revealing partial co-localization in hippocampal neurons and less frequent occurrence in cortical neurons.
The SUMOylation machinery is a regulator of neuronal activity and synaptic plasticity. It is composed of SUMO isoforms and specialized enzymes named E1, E2 and E3 SUMO ligases. Recent studies have high-lighted how SUMO isoforms and E2 enzymes localize with synaptic markers to support previous functional studies but less information is available on E3 ligases. PIAS proteins - belonging to the protein inhibitor of activated STAT (PIAS) SUMO E3-ligase family - are the best-characterized SUMO E3-ligases and have been linked to the formation of spatial memory in rodents. Whether however they exert their function co-localizing with synaptic markers is still unclear. In this study, we applied for the first time structured illumination microscopy (SIM) to PIAS ligases to investigate the co-localization of PIAS1 and PIAS3 with synaptic markers in hippocampal and cortical murine neurons. The results indicate partial co-localization of PIAS1 and PIAS3 with synaptic markers in hippocampal neurons and much rarer occurrence in cortical neurons. This is in line with previous super-resolution reports describing the co-localization with synaptic markers of other components of the SUMOylation machinery.
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