Journal
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
Volume 247, Issue 9, Pages 2183-2192Publisher
SPRINGER
DOI: 10.1007/s00217-021-03778-y
Keywords
mtDNA; PCR-RFLP; Species-specific primers PCR; Animal species; Authentication
Categories
Funding
- Ministry of Higher Education and Scientific Research of Tunisia
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The study recommends using PCR-RFLP and PCR techniques for meat species detection, detecting multiple animal species and identifying 10 animal species in daily meat analysis.
The traceability of meat origin has become a necessity to safeguard consumer's confidence in commercial meat products. Our study recommends a meat species detection using qualitative approach based on PCR-RFLP and species-specific primers PCR. Here, we targeted a 359 bp fragment of the mitochondrial cytochrome b gene amplified by PCR using universal primers followed by three enzymatic digestions. Seven animal species including dromedary, rabbit, goat, turkey, rat, donkey and pork, have been efficiently detected in pure and mixed samples. The combination of PCR-RFLP and triplex PCR assays offers, in addition, the identification of chicken, dog and cat species in meat. In conclusion, by the mean of PCR-based techniques using universal primers followed by enzymatic digestion and multiplex primer-specific approach, we developed an extensible protocol by which we identified 10 animal species that could be integrated in meat analysis daily routine.
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