Journal
EPIDEMIOLOGY AND INFECTION
Volume 149, Issue -, Pages -Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0950268821002090
Keywords
Cyclospora cayetanensis; genotyping; clusters; outbreak; cyclosporiasis
Funding
- Centers for Disease Control and Prevention Office of Advanced Molecular Detection
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Cyclosporiasis is an illness caused by the food-borne parasite Cyclospora cayetanensis, with an increase in cases leading to the development of genotyping tools for outbreak investigations. The CDC's system, based on deep amplicon sequencing and machine learning, has been improved and shows excellent sensitivity and specificity, with the potential to become the basis of a nationwide genotyping network.
Cyclosporiasis is an illness characterised by watery diarrhoea caused by the food-borne parasite Cyclospora cayetanensis. The increase in annual US cyclosporiasis cases led public health agencies to develop genotyping tools that aid outbreak investigations. A team at the Centers for Disease Control and Prevention (CDC) developed a system based on deep amplicon sequencing and machine learning, for detecting genetically-related clusters of cyclosporiasis to aid epidemiologic investigations. An evaluation of this system during 2018 supported its robustness, indicating that it possessed sufficient utility to warrant further evaluation. However, the earliest version of CDC's system had some limitations from a bioinformatics standpoint. Namely, reliance on proprietary software, the inability to detect novel haplotypes and absence of a strategy to select an appropriate number of discrete genetic clusters would limit the system's future deployment potential. We recently introduced several improvements that address these limitations and the aim of this study was to reassess the system's performance to ensure that the changes introduced had no observable negative impacts. Comparison of epidemiologically-defined cyclosporiasis clusters from 2019 to analogous genetic clusters detected using CDC's improved system reaffirmed its excellent sensitivity (90%) and specificity (99%), and confirmed its high discriminatory power. This C. cayetanensis genotyping system is robust and with ongoing improvement will form the basis of a US-wide C. cayetanensis genotyping network for clinical specimens.
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