Journal
ENVIRONMENTAL SCIENCE & TECHNOLOGY
Volume 55, Issue 17, Pages 11894-11905Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.est.1c03457
Keywords
dietary Se-Met; zebrafish; neurotransmitters; behavioral toxicity; signal pathways
Categories
Funding
- National Natural Science Foundation of China [41877366, 41807352, U1901218]
- National Key Research and Development Program of China [2018YFC1801200]
- Guangdong Basic and Applied Basic Research Foundation [2019A1515110789, 2020A1515010508]
- China Postdoctoral Science Foundation [2019 M662956]
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The study revealed that dietary seleno-L-methionine can cause brain damage and hormonal imbalance in fish, affecting neurotransmission. Fish in the high treatment group showed significantly altered swimming behavior in a novel environment.
Elevated concentrations of dietary selenium (Se) cause abnormalities and extirpation of fish inhabiting in Se-contaminated environments. However, its effect on fish behavior and the underlying mechanisms remain largely unknown. In this study, two-month-old zebrafish (Danio rerio) was fed seleno-L-methionine (Se-Met) at environmentally relevant concentrations (i.e., control (2.61), low (5.43), medium (12.16), and high (34.61) mu g Se/g dry weight (dw), respectively, corresponding to the C, L, M, and H treatments) for 60 days. Targeted metabolomics, histopathological, and targeted transcriptional endpoints were compared to behavioral metrics to evaluate the effects of dietary exposure to Se-Met. The results showed that the levels of total Se and malondialdehyde in fish brains were increased in a dose-dependent pattern. Meanwhile, mitochondrial damages and decreased activities of the mitochondria respiratory chain complexes were observed in the neurons at the M and H treatments. In addition, dietary Se-Met affected neurotransmitters, metabolites, and transcripts of the genes associated with the dopamine, serotonin, gamma-aminobutyric acid, acetylcholine, and histamine signaling pathways in zebrafish brains at the H treatments. The total swimming distance and duration in the Novel Arm were lowered in fish from the H treatment. This study has demonstrated that dietary Se-Met affects the ultrastructure of the zebrafish brain, neurotransmitters, and associated fish behaviors and may help enhance adverse outcome pathways for neurotransmitter-behavior key events in zebrafish.
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