Synchrotron-Based Imaging Reveals the Fate of Selenium in Striped Marsh Frog Tadpoles

Synchrotron-based X-ray fluorescence microscopy (XFM) coupled with X-ray absorption near-edge structure (XANES) imaging was used to study selenium (Se) biodistribution and speciation in Limnodynastes peronii tadpoles. Tadpoles were exposed to dissolved Se (30 mu g/L) as selenite (Se-IV) or selenate (Se-VI) for 7 days followed by 3 days of depuration. High-resolution elemental maps revealed that Se partitioned primarily in the eyes (specifically the eye lens, iris, and retinal pigmented epithelium), digestive and excretory organs of Se-IV-exposed tadpoles. Speciation analysis confirmed that the majority of accumulated Se was converted to organo-Se. Multielement analyses provided new information on Se colocalization and its impact on trace element homeostasis. New insights into the fate of Se on a whole organism scale contribute to our understanding of the mechanisms and risks associated with Se pollution.

Automated summary

Synchrotron-based XFM and XANES imaging were used to study Se biodistribution and speciation in Limnodynastes peronii tadpoles. Results showed that Se primarily accumulated in the eyes, digestive, and excretory organs, with the majority converted to organo-Se.