Seasonal diet-based resistance to anticoagulant rodenticides in the fossorial water vole (Arvicola amphibius)

Anticoagulant rodenticides (AR) resistance has been defined as a major loss of efficacy due to the presence of a strain of rodent with a heritable and commensurately reduced sensitivity to the anticoagulant. The mechanism that supports this resistance has been identified as based on mutations in the Vkorc1 gene leading to severe resistance in rats and mice. This study evaluates the validity of this definition in the fossorial water vole and explores the possibility of a non-genetic diet-based resistance in a strict herbivorous rodent species. Genetic support was explored by sequencing the Vkorc1 gene and the diet-based resistance was explored by the dosing of vitamins K in liver of voles according to seasons. From a sample of 300 voles, only 2 coding mutations, G71R and S149I, were detected in the Vkorc1 gene in the heterozygous state with low allele frequencies (0.5-1%). These mutations did not modify the sensitivity to AR, suggesting an absence of genetic Vkorc1-based resistance in the water vole. On the contrary, vitamin K1 was shown to be 5 times more abundant in the liver of the water vole compared to rats. This liver concentration was shown to seasonally vary, with a trough in late winter and a peak in late spring/early summer related to the growth profile of grass. This increase in concentration might be responsible for the increased resistance of water voles to AR. This study highlights a non-genetic, diet-related resistance mechanism in rodents to AR. This diet-based resistance might explain the different evolution of the Vkorc1 gene in the fossorial water vole compared to rats and mice.

Automated summary

The study found that there is no genetic basis for anticoagulant rodenticides resistance in fossorial water voles, but rather a diet-based resistance mechanism. Only two coding mutations were detected in the Vkorc1 gene in a small percentage of the voles, which did not alter their sensitivity to anticoagulant rodenticides. Instead, the seasonal variation in vitamin K1 concentration in the voles' liver was found to be responsible for their increased resistance.