An integrated approach for assessing the in vitro and in vivo redox-related effects of nanomaterials

Over the last few decades, nanotechnology has risen to the forefront of both the research and industrial interest, resulting in the manufacture and utilization of various nanomaterials, as well as in their integration into a wide range of fields. However, the consequent elevated exposure to such materials raises serious concerns regarding their effects on human health and safety. Existing scientific data indicate that the induction of oxidative stress, through the excessive generation of Reactive Oxygen Species (ROS), might be the principal mechanism of exerting their toxicity. Meanwhile, a number of nanomaterials exhibit antioxidant properties, either intrinsic or resulting from their functionalization with conventional antioxidants. Considering that their redox properties are implicated in the manifestation of their biological effects, we propose an integrated approach for the assessment of the redox-related activities of nanomaterials at three biological levels (in vitro-cell free systems, cell cultures, in vivo). Towards this direction, a battery of translational biomarkers is recommended, and a series of reliable protocols are presented in detail. The aim of the present approach is to acquire a better understanding with respect to the biological actions of nanomaterials in the interrelated fields of Redox Biology and Toxicology.

Automated summary

Nanotechnology has become a forefront of research and industrial interest in recent decades, but concerns have been raised about the impacts of nanomaterials on human health and safety. Scientific data suggest that oxidative stress induced by Reactive Oxygen Species (ROS) may be the main mechanism of toxicity, although some nanomaterials also exhibit antioxidant properties. An integrated approach using translational biomarkers and reliable protocols is recommended for the assessment of redox-related activities of nanomaterials at different biological levels.