Journal
FEBS LETTERS
Volume 590, Issue 3, Pages 396-407Publisher
WILEY
DOI: 10.1002/1873-3468.12064
Keywords
hBMSCs; miR-33a-5p; osteogenesis; SATB2; TNF-alpha
Funding
- National Natural Science Foundation of China [31470946, 81170988]
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miRNAs play a number of roles in bone, including mediating the pathological effects of inflammation. Here, we found that miR-33a-5p expression was significantly increased after TNF-alpha treatment during BMP-2-induced osteogenic differentiation of hBMSCs. Luciferase reporter assays and western blotting demonstrated that special AT-rich sequence-binding protein 2 (SATB2) is a target of miR-33a-5p. Moreover, we show that BMP-2 induces SATB2 expression by interacting with SATB2 directly via the BMP-2-RUNX2 pathway. However, TNF-a first decreases SATB2 expression by inhibiting miR33a- 5p degradation. We thus conclude that miR-33a-5p plays a central role in this complex regulatory network. These findings will help to understand the regulatory role of miR-33a-5p in the inflammatory process.
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