Journal
FEBS LETTERS
Volume 591, Issue 1, Pages 5-15Publisher
WILEY-BLACKWELL
DOI: 10.1002/1873-3468.12507
Keywords
amino acid transporter; Arabidopsis; export; vacuole; yeast
Funding
- Ministry of Education, Culture, Sports, Science, and Technology of Japan
- Grants-in-Aid for Scientific Research [15K07095] Funding Source: KAKEN
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Amino acids stored in the vacuoles are exported to the cytosol mainly for protein synthesis; however, the molecular identity of vacuolar amino acid exporters remains obscure in plants. Here, we demonstrate that the heterologous expression of AtAVT3 genes, Arabidopsis homologs of AVT3 and AVT4 encoding vacuolar amino acid exporters in yeast, reduces vacuolar amino acid levels in the avt3 Delta avt4 Delta yeast cells. In vitro experiments revealed that C-14-labeled Ala and Pro are exported from vacuolar membrane vesicles by AtAvt3A in an ATP-dependent manner. In Arabidopsis, AtAvt3A fused with green fluorescent protein localizes to the vacuolar membrane. We propose that AtAVT3 family represents the long sought-for vacuolar amino acid exporters in plants.
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