Journal
ENVIRONMENTAL POLLUTION
Volume 278, Issue -, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.envpol.2021.116821
Keywords
Homeostasis; Next-generation sequencing; Persistent organic pollutants; Fish; Metagenomics; 16s rRNA metagenetics
Categories
Funding
- Global Water Futures program, Canada First Research Excellence Fund [419205]
- Global Water Futures program
- Banting Post-Doctoral Fellowship
- Canada Research Chairs Program of the Natural Sciences and Engineering Research Council of Canada (NSERC)
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The study showed that environmentally-relevant concentrations of BaP can alter the diversity of the gut microbiome and community network connectivity. Exposure to BaP reduced alpha diversity at the greatest exposure concentrations, but also altered community composition and reduced network complexity of the microbial community.
The microbiome has been described as an additional host organ with well-established beneficial roles. However, the effects of exposures to chemicals on both structure and function of the gut microbiome of fishes are understudied. To determine effects of benzo[a]pyrene (BaP), a model persistent organic pollutant, on structural shifts of gut microbiome in juvenile fathead minnows (Pimephales promelas), fish were exposed ad libitum in the diet to concentrations of 1,10,100, or 1000 mg BaP g(-1) food, in addition to a vehicle control, for two weeks. To determine the link between exposure to BaP and changes in the microbial community, concentrations of metabolites of BaP were measured in fish bile and 16S rRNA amplicon sequencing was used to evaluate the microbiome. Exposure to BaP only reduced alpha diversity at the greatest exposure concentrations. However, it did alter community composition assessed as differential abundance of taxa and reduced network complexity of the microbial community in all exposure groups. Results presented here illustrate that environmentally-relevant concentrations of BaP can alter the diversity of the gut microbiome and community network connectivity. (C) 2021 Elsevier Ltd. All rights reserved.
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