Journal
ENVIRONMENTAL MICROBIOLOGY
Volume 23, Issue 9, Pages 5487-5504Publisher
WILEY
DOI: 10.1111/1462-2920.15693
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Funding
- Italian Ministry for Education, University and Research [RBFR10LHD1_002, 20177J5Y3P]
- Italian Cystic FibrosisResearch Foundation [FFC 17/2018]
- Grant of Excellence Departments, MIUR-Italy [COMMI 314 -337 LEGGE 232/2016]
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DksA1 and DksA2 play critical roles in P. aeruginosa environmental adaptation, largely interchangeable with each other. While most genes' expression levels are restored by either dksA1 or dksA2, a small subset of genes seems to be preferentially complemented by one of them.
The stringent response regulator DksA plays a key role in Gram negative bacteria adaptation to challenging environments. Intriguingly, the plant and human pathogen Pseudomonas aeruginosa is unique as it expresses two functional DksA paralogs: DksA1 and DksA2. However, the role of DksA2 in P. aeruginosa adaptive strategies has been poorly investigated so far. Here, RNA-Seq analysis and phenotypic assays showed that P. aeruginosa DksA1 and DksA2 proteins are largely interchangeable. Relative to wild type P. aeruginosa, transcription of 1779 genes was altered in a dksA1 dksA2 double mutant, and the wild type expression level of >= 90% of these genes was restored by in trans complementation with either dksA1 or dksA2. Interestingly, the expression of a small sub-set of genes seems to be preferentially or exclusively complemented by either dksA1 or dksA2. In addition, evidence has been provided that the DksA-dependent regulation of virulence genes expression is independent and hierarchically dominant over two major P. aeruginosa regulatory circuits, i.e., quorum sensing and cyclic-di-GMP signalling systems. Our findings support the prominent role of both DksA paralogs in P. aeruginosa environmental adaptation.
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