Journal
FEBS LETTERS
Volume 590, Issue 16, Pages 2619-2628Publisher
WILEY
DOI: 10.1002/1873-3468.12312
Keywords
baicalein; liverworts; O-methyltransferase; Plagiochasma appendiculatum; scutellarein
Funding
- National Natural Science Foundation of China [31170280, 31370330]
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Oroxylin A and hispidulin, compounds which are abundant in both Scutellaria and liverwort species, are important lead compounds for the treatment of ischemic cerebrovascular disease. Their enzymatic synthesis requires an O-methyltransferase able to interact with the related flavonoid's 6-OH group, but such an enzyme has yet to be identified in plants. Here, the gene encoding an O-methyltransferase (designated PaF6OMT) was isolated from the liverwort species Plagiochasma appendiculatum. A test of alternative substrates revealed that its strongest preferences were baicalein and scutellarein, which were converted into, respectively, oroxylin A and hispidulin. Allowed a sufficient reaction time, the conversion rate of these two substrates was, respectively, 90% and 100%. PaF6OMT offers an enzymatic route to the synthesis of oroxylin A and hispidulin.
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