Journal
FEBS LETTERS
Volume 590, Issue 14, Pages 2146-2157Publisher
WILEY-BLACKWELL
DOI: 10.1002/1873-3468.12227
Keywords
BLUF; C terminus; dark recovery kinetics; photoreceptors; Stenotrophomonas sp SKA14
Funding
- UGC
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BLUF domains are flavin-based photoreceptors which receive the blue light signal and are involved in the sensory transduction. We report a short BLUF photoreceptor (SnfB) in Stenotrophomonas sp. We have investigated photodynamic properties of C terminus truncated and several mutated SnfB proteins. Deletion of the extended C-terminal residues alters the thermal recovery kinetics and also affects the integrity of the SnfB protein. Mutagenesis studies demonstrated that the conserved residues within and outside the flavin-binding pocket also regulates the photocycle properties of the protein. These studies suggest that the C-terminal residues outside the BLUF domain can tune the photodynamic properties of the BLUF protein.
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