Investigation of selected parameters of capillary zone electrophoresis method for analysis of isolates of outer membrane vesicles

The capillary zone electrophoresis (CZE) has recently been proposed by our group as a novel technique for outer membrane vesicles (OMVs) characterization (J. Chromatography 1621 (2020) 461047). In present work the impact of selected parameters of CZE method on OMVs isolates analysis was assessed. It was shown that the extension of sample injection plug length significantly improves the detectability of macromolecular aggregates in CZE. Moreover, a negligible adsorption of OMVs to both uncoated and polymer-modified (poly(DMA-GMA-MAPS)) capillary walls was proven. Finally, the relaxation effect as well as deformation/polarization of vesicles were demonstrated to affect OMVs electrophoretic mobility. The significance of these findings was discussed.

Automated summary

The study evaluated the impact of selected parameters of CZE method on OMVs isolate analysis, finding that extending the sample injection plug length significantly improves the detectability of macromolecular aggregates in CZE, with negligible adsorption of OMVs to capillary walls. Additionally, the relaxation effect and deformation/polarization of vesicles were shown to affect OMVs electrophoretic mobility.