4.5 Article

Time-resolved particle image velocimetry analysis and computational modeling of transient optically induced electrothermal micro vortex

Journal

ELECTROPHORESIS
Volume 42, Issue 23, Pages 2483-2489

Publisher

WILEY
DOI: 10.1002/elps.202100104

Keywords

Colloid; Electrokinetic; Electrothermal; Micro-Manipulation

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This study investigates the behavior and properties of synthetic microparticles and biological cells using opto-electric heating and microfluidic techniques. It explores the real-time tuning of vortex characteristics in REP micro vortex and analyzes the process through experiments and computational modeling, while also discussing the limitations of using superposition in custom-shaped traps.
Trapping, sorting, transportation, and manipulation of synthetic microparticles and biological cells enable investigations in their behavior and properties. Microfluidic techniques like rapid electrokinetic patterning (REP) provide a non-invasive means to probe into the nature of these micro and nanoparticles. The opto-electrically induced nature of a REP micro vortex allows tuning of the trap characteristics in real-time. In this work, we studied the effects of transient optical heating on the induced electrothermal vortex using micro-particle image velocimetry (mu-PIV) and computational modeling. A near infra-red (980 nm) laser beam was focused on a colloidal suspension of 1 mu m polystyrene beads sandwiched between two parallel-plate electrodes. The electrodes were subjected to an AC current. The laser spot was scanned back-and-forth in a line, at different frequencies, to create the transient vortex. This phenomenon was also studied with a computational model made using COMSOL Multiphysics. We visualize fluid flow in custom-shaped REP traps by superposing multiple axisymmetric (spot) vortices and discuss the limitations of using superposition in dynamically changing traps.

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