4.2 Article

Selective Stimulation of a Target Neuron in Micropatterned Neuronal Circuits Using a Pair of Needle Electrodes

Journal

ELECTROCHEMISTRY
Volume 89, Issue 4, Pages 348-354

Publisher

ELECTROCHEMICAL SOC JAPAN
DOI: 10.5796/electrochemistry.21-00032

Keywords

Neuron; Two Needle Electrodes; Selective Stimulation; LTspice

Funding

  1. JSPS KAKENHI [18K19026, 18H03325, 20K19925]
  2. Waseda University Grant for Special Research Projects [BARE004443]
  3. Cooperative Research Project Program of the RIEC, Tohoku University
  4. Japan Science and Technology Agency [JPMJCR14F3]
  5. Project of Creation of Life Innovation Materials for Interdisciplinary and International Researcher Development of the Ministry of Education, Culture, Sports, Science and Technology (MEXT), Japan
  6. Grants-in-Aid for Scientific Research [18K19026, 20K19925, 18H03325] Funding Source: KAKEN

Ask authors/readers for more resources

Neurostimulation is a crucial technique for manipulating local neuronal circuits, but current methods have limitations in terms of precision and long-term monitoring. A new approach using two needle electrodes and micropatterning techniques shows promise for selectively targeting and stimulating single neurons. Results indicate that the method can stimulate a target neuron selectively by adjusting the position and voltage steepness of the two needle electrodes, making it suitable for long-term monitoring of neuronal circuit activity.
Neurostimulation is an essential technique to trigger and modulate the spatiotemporal activity of local neuronal circuits. Current stimulation methods have a trade-off relationship among aiming precision, temporal resolution, and noninvasiveness, making it difficult to stimulate and monitor a single target neuron for a long term. Here, we show that a method using two needle electrodes in combination of micropatterning techniques provides new possibilities for targeting and stimulating a single neuron selectively. Results of physiological experiments as well as analog circuit simulation reveal that two needle electrodes can stimulate a target neuron selectively by placing the two needle electrodes in proximity to and to straddle the target neuron, and that the steepness of voltage applied to two needle electrodes is important for the target neuron to fire at a low voltage. The proposed method enables a noninvasive stimulation suitable for measuring long-term activity of local neuronal circuits. (C) The Author(s) 2021. Published by ECSJ.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.2
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available