Potential role of lysine succinylation in the response of moths to artificial light at night stress

Artificial light at night (ALAN) is a widespread environmental pollutant and stressor. Many nocturnal insects have been shown to experience ALAN stress. However, few studies have been conducted to uncover the mechanism by which nocturnal insects respond to ALAN stress. Previous studies suggest that lysine succinylation (Ksuc) is a potential mechanism that coordinates energy metabolism and antioxidant activity under stressful conditions. Mythimna separata (Walker) (M. separata) is a nocturnal insect that has been stressed by ALAN. In this study, we quantified the relative proteomic Ksuc levels in ALAN-stressed M. separata. Of the 466 identified Ksucmodified proteins, 103 were hypersuccinylated/desuccinylated in ALAN-stressed moths. The hypersuccinylated/desuccinylated proteins were shown to be involved in various biological processes. In particular, they were enriched in metabolic processes, reactive oxygen species (ROS) homeostasis and the neuromuscular system. Furthermore, we demonstrated that Ksuc might affect moth locomotion by intervening with and coordinating these systems under ALAN stress. These findings suggest that Ksuc plays a vital role in the moth response to ALAN stress and moth locomotion behavior and provide a new perspective on the impact of ALAN on nocturnal insect populations and species communities.

Automated summary

This study quantified the relative proteomic Ksuc levels in ALAN-stressed M. separata, finding that some proteins were hypersuccinylated/desuccinylated in response to ALAN stress and involved in metabolic processes, ROS homeostasis, and the neuromuscular system, influencing moth locomotion. These findings suggest that Ksuc plays a crucial role in the moth response to ALAN stress and locomotion behavior, offering a new perspective on the impact of ALAN on nocturnal insect populations and species communities.