Journal
DEVELOPMENTAL CELL
Volume 56, Issue 11, Pages 1677-+Publisher
CELL PRESS
DOI: 10.1016/j.devcel.2021.05.001
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Funding
- Deutsche Forschungsgemeinschaft [CRC1324, 331,351,713, CRC-TR209, 314905040, CRC1366, 39404578]
- European Research Council Advanced Grant AngioMature'' [787181]
- State of Baden-Wurttemberg Foundation special program Angioformatics Single Cell Platform
- European Research Council (ERC) [787181] Funding Source: European Research Council (ERC)
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This study combines spatial cell sorting with transcriptomics and quantitative proteomics/phosphoproteomics to establish a spatially resolved proteome landscape of liver endothelium, providing fundamental insight into the spatial organization of liver endothelial cell signaling. The research identifies Tie receptor signaling as a selective and specific regulator of vascular Wnt activity, controlling hepatocyte function during liver regeneration. The spatial sorting strategy may be universally adaptable for multiomic analyses of cellular populations defined by scRNA-seq.
Single-cell transcriptomics (scRNA-seq) has revolutionized the understanding of the spatial architecture of tissue structure and function. Advancing the ``transcript-centric'' view of scRNA-seq analyses is presently restricted by the limited resolution of proteomics and genome-wide techniques to analyze post-translational modifications. Here, by combining spatial cell sorting with transcriptomics and quantitative proteomics/phosphoproteomics, we established the spatially resolved proteome landscape of the liver endothelium, yielding deep mechanistic insight into zonated vascular signaling mechanisms. Phosphorylation of receptor tyrosine kinases was detected preferentially in the central vein area, resulting in an atypical enrichment of tyrosine phosphorylation. Prototypic biological validation identified Tie receptor signaling as a selective and specific regulator of vascular Wnt activity orchestrating angiocrine signaling, thereby controlling hepatocyte function during liver regeneration. Taken together, the study has yielded fundamental insight into the spatial organization of liver endothelial cell signaling. Spatial sorting may be employed as a universally adaptable strategy for multiomic analyses of scRNA-seq-defined cellular (sub)-populations.
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