Expression of CD9 on porcine lymphocytes and its relation to T cell differentiation and cytokine production

In this work, we report on two novel monoclonal antibodies, specific for porcine CD9. CD9 is a tetraspanin that is expressed on a wide variety of cells. We phenotyped porcine immune cell subsets and found that CD9 was expressed on all monocytes as well as a subset of B cells. CD9 was variably expressed on T cells, with CD4 T cells containing the highest frequency of CD9+ cells. CD9 expression positively correlated with the frequency of central memory CD4 T cells in ex vivo PBMC. Therefore, we proceeded to explore CD9 as a marker of T cell function. Here we observed that CD9 was expressed on the vast majority of long-lived influenza A virus-specific effector cells that retained the capacity for cytokine production in response to in vitro recall antigen. Therefore, the new antibodies enable the detection of a cell surface molecule with functional relevance to T cells. Considering the importance of CD9 in membrane remodelling across many cell types, they will also benefit the wider field of swine biomedical research.

Automated summary

In this study, two novel monoclonal antibodies specific for porcine CD9 were reported. CD9 expression was found on a wide range of porcine immune cell subsets, with a correlation between CD9 expression and central memory CD4 T cells. The antibodies enable the detection of a cell surface molecule with functional relevance to T cells, benefiting swine biomedical research.