4.3 Article

OMIP 078: A 31-parameter panel for comprehensive immunophenotyping of multiple immune cells in human peripheral blood mononuclear cells

Journal

CYTOMETRY PART A
Volume 99, Issue 9, Pages 893-898

Publisher

WILEY
DOI: 10.1002/cyto.a.24490

Keywords

aging; immune cells; multi-color flow cytometry

Funding

  1. Japan Society for the Promotion of Science [JP19k21601, JP20H03728, JP20K22774]
  2. Japan Agency for Medical Research and Development [JP20fk0410023, JP21fk0210057, JP20fk0108141, JP21fk0410040, JP21wm0325015]
  3. Nippon Becton Dickinson Company, Ltd

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This 31-parameter panel is designed to measure multiple immune cell populations in human peripheral blood mononuclear cells, providing insights into immune dynamics and single-cell changes associated with aging and diseases. It includes markers to differentiate each cell population's differentiation status and is useful for studying infectious and autoimmune diseases where patient samples are limited and require detailed information.
This 31-parameter panel was developed for simultaneously measuring multiple immune cell populations including T cells, B cells, natural killer cells, dendritic cells, monocytes, and hematopoietic progenitor cells in human peripheral blood mononuclear cells. This panel enables the capture of individual immune dynamics and assessments of single-cell changes in the immune system that are associated with aging and diseases. This panel includes markers to separate the differentiation status of each cell population and might be applicable to studies of infectious and autoimmune diseases, as patient samples are usually limited in volume and require an analysis system that provides a relatively large amount of information.

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