Qipengyuania soli sp. nov., Isolated from Mangrove Soil

A novel Gram-stain-negative, non-motile, and rod-shaped bacterial strain, designated as 6D36(T), was isolated from mangrove soil and characterized by using a polyphasic taxonomic approach. Strain 6D36(T) was found to grow at 10-37 degrees C (optimum, 28 degrees C), at pH 6.0-9.0 (optimum, 7.0) and in 0-8% (w/v) NaCl (optimum, 3%). The predominant cellular fatty acids of strain 6D36(T) were summed feature 8 (C-19:1 omega 7c and/or C-18:1 omega 6c) and C-17:1 omega 6c; the major polar lipids were diphosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, and sphingoglycolipid; the sole respiratory quinone was Q-10. The phylogenetic analysis based on 16S rRNA gene sequences showed that strain 6D36(T) fell into the genus Qipengyuania and was closely related to Erythrobacter mangrovi MCCC 1K03690(T) (98.5%), Qipengyuania citrea CGMCC 1.8703(T) (97.6%), and Qipengyuania pelagi JCM 17468(T) (97.4%). The phylogenomic analysis indicated that strain 6D36(T) formed an independent branch distinct from reference-type strains of species within this genus. The digital DNA-DNA hybridization and average nucleotide identity values between strain 6D36(T) and the three type strains above were, respectively, 20.2-21.3% and 79.5-81.5%, of which were far below their respective threshold for species definition, implying that the strain represents a novel genospecies. The genomic DNA G + C content was 63.3%. Based on phenotypic and genotypic characteristics, strain 6D36(T) is concluded to represent a novel species of the genus Qipengyuania, for which the name Qipengyuania soli sp. nov., is proposed. The type strain of the species is 6D36(T) (= GDMCC 1.1977(T) = KCTC 82333(T)).

Automated summary

Strain 6D36(T) was a novel Gram-stain-negative, non-motile, and rod-shaped bacterium isolated from mangrove soil, with optimal growth at 28 degrees C, pH 7.0, and 3% NaCl. Phylogenetic analysis showed it belonged to the genus Qipengyuania and formed an independent branch distinct from other species within this genus.