Journal
CURRENT MICROBIOLOGY
Volume 78, Issue 8, Pages 3230-3238Publisher
SPRINGER
DOI: 10.1007/s00284-021-02583-w
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- Shahed University
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The study used FISH and PCR to determine prokaryotic diversity in Urmia Salt Lake. It found that the prokaryote cell populations were more diverse and abundant than expected in this hypersaline environment, with bacteria forming the metabolically active part of the microbial population. Molecular approaches revealed different aspects of the microbiota in this extreme environment.
In this study, fluorescence in situ hybridization (FISH) and PCR-amplified fragments of the 16SrDNA gene were used to determine prokaryotes diversity in Urmia Salt Lake. Prokaryote cell population in Urmia lake range from 3.1 +/- 0.3 x 10(6), 2 +/- 0.2 x 10(8), 4 +/- 0.3 x 10(8), and 1.8 +/- 0.2 x 10(8) cells ml(-1) for water, soil, sediment, and salt samples by DAPI (4, 6-diamidino-2-phenylindole) direct count, respectively. The proportion of bacteria and archaea in the samples determinable by FISH ranged between 36.1 and 55% and 48.5 and 55.5%, respectively. According to the DGGE method, some bands were selected and separated from the gel, then amplified and sequenced. The results of sequences were related to two phyla Proteobacteria (16.6%) and Bacteroidetes (83.3%), which belonged to four genera Salinibacter, Mangroviflexus, Pseudomonas, and Cesiri-bacter, and the archaeal sequences were related to Euryarchaeota phyla and three genera Halonotius, Haloquadraturn, and Halorubrum. According to our results, it seems that prokaryotic populations in this hypersaline environment are more diverse than expected, and bacteria are so abundant and diverse and form the metabolically active part of the microbial population inhabiting this extreme environment. Molecular dependent and independent approaches revealed a different aspect of this environment microbiota.
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