Journal
CRYOBIOLOGY
Volume 102, Issue -, Pages 42-55Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.cryobiol.2021.07.013
Keywords
Mitochondria; Hypothermia; Mitochondrial fission; Drp1; TRPV1
Categories
Funding
- NIH [P01 HL112730]
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Hypothermia is a valuable clinical tool in mitigating ischemic consequences, primarily through reducing metabolism and inhibiting mitochondrial fission. It leads to elongation of cardiac mitochondria and suppressed oxygen consumption, potentially through decreased expression of key proteins like TRPV1 and pDrp1S616.
Hypothermia is a valuable clinical tool in mitigating against the consequences of ischemia in surgery, stroke, cardiac arrest and organ preservation. Protection is afforded principally by a reduction of metabolism, manifesting as reduced rates of oxygen uptake, preservation of ATP levels, and a curtailing of ischemic calcium overload. The effects of non-ischemic hypothermic stress are relatively unknown. We sought to investigate the effects of clinically mild-to-severe hypothermia on mitochondrial morphology, oxygen consumption and protein expression in normoxic hearts and cardiac cells. Normoxic perfusion of rat hearts at 28-32 degrees C was associated with inhibition of mitochondrial fission, evi-denced by a reduced abundance of the active phosphorylated form of the fission receptor Drp1 (pDrp1(S616)). Abundance of the same residue was reduced in H9c2 cells subjected to hypothermic culture (25-32 degrees C), in addition to a reduced abundance of the Drp1 receptor MFF. Hypothermia-treated H9c2 cardiomyocytes exhibited elongated mitochondria and depressed rates of mitochondrial-associated oxygen consumption, which persisted upon rewarming. Hypothermia also promoted a reduction in mRNA expression of the capsaicin receptor TRPV1 in H9c2 cells. When normothermic H9c2 cells were transfected with TRPV1 siRNA we observed reduced pDrp1(S616) and MFF abundance, elongated mitochondria, and reduced rates of mitochondrial-associated oxygen consumption, mimicking the effects of hypothermic culture. In conclusion hypothermia promoted elongation of cardiac mitochondria via reduced pDrp1S616 abundance which was also associated with suppression of cellular oxygen consumption. Silencing of TRPV1 in H9c2 cardiomyocytes reproduced the morphological and respirometric phenotype of hypothermia. This report demonstrates a novel mechanism of cold-induced inhibition of mitochondrial fission.
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