Alternaria toxins-Still emerging?

Alternaria molds are known to cause the contamination of food with their secondary metabolites, a chemically very heterogeneous group of compounds. Yet, after decades of research on the occurrence and the toxicity of Alternaria toxins in academia, no regulation has been implemented yet, thus leaving these potential food contaminants in the status of so-called emerging mycotoxins. However, research on this topic has been far from static, leading to the European Food Safety Authority repeatedly calling for more data on the occurrence and toxicity of genotoxic metabolites such as alternariol (AOH) and its monomethyl ether (AME). To give an overview on recent developments in the field, this comprehensive review summarizes published data and addresses current challenges arising from the chemical complexity of Alternaria's metabolome, mixture effects and the emergence of novel biological targets like cell membranes or the interaction with different receptors. Besides toxicodynamics, we review recent research on toxicokinetics, including the first in vivo studies which incorporated the rarely investigated-but highly genotoxic-perylene quinones. Furthermore, a particular focus lies on the advances of liquid chromatography/tandem mass spectrometry (LC-MS/MS)-based analytical tools for determining a broader spectrum of Alternaria toxins including modified/masked forms and assessing exposure via human biomonitoring (HBM).

Automated summary

This review summarizes the current research on the contamination and toxicity of Alternaria and its metabolites in food, highlighting the challenges posed by their chemical complexity and mixture effects, as well as the recent advances in toxicokinetics research, including in vivo studies of genotoxic pollutants such as perylene quinones. Additionally, it discusses the progress in the development of analytical tools based on liquid chromatography/tandem mass spectrometry for a broader spectrum of Alternaria toxins and assessing exposure through human biomonitoring.