Hypoxia-induced oxidative stress and transcriptome changes in the mud crab (Scylla paramamosain)

Mud crab (Scylla paramamosain) is an economically important cultured species in China. Hypoxia is a major environmental stressor during mud crab culture. In the present study, we investigated the oxidative stress and transcriptome changes in the gills of mud crab after intermediate hypoxia stress with dissolved oxygen (DO) 3.0 +/- 0.2 mg/L (named as DO3) and acute hypoxia stress with DO 1.0 +/- 0.2 mg/L (named as DO1) for 0, 3, 6, 12 and 24 h. The superoxide dismutase (SOD) activity of DO1 increased significantly at 3, 6 and 24 h after hypoxia stress, while SOD activity of DO3 increased significantly at 6 and 24 h. The total antioxidant capacity (T-AOC) increased significantly at 6, 12 and 24 h after hypoxia stress. The malondialdehyde (MDA) concentration of DO1 increased significantly at 6, 12 and 24 h after hypoxia stress, while MDA concentration of DO3 only increased significantly at 6 h. The lactate dehydrogenase (LDH) activity of DO1 increased significantly at 3, 6, 12 and 24 h after hypoxia stress, while LDH activity of DO3 increased significantly at 12 and 24 h. Transcriptomic analysis was conducted at 24 h of gill tissues after hypoxia stress. A total of 1052 differentially expressed genes (DEGs) were obtained, including 394 DEGs between DO1 and DO3, 481 DEGs between DO1 and control group, 177 DEGs between DO3 and control group. DEGs were enriched in the pathways related to metabolism, immune functions, ion transport, and signal transduction. Transcriptional analysis showed that glycolysis and tricarboxylic acid cycle genes were the key factors in regulating the adaptation of mud crab to hypoxia stress.

Automated summary

The mud crab is an economically important cultured species in China, and hypoxia is a major environmental stressor during its culture. The study found that the activities of SOD, T-AOC, and LDH in the gills of mud crab increased significantly after hypoxia stress, while MDA concentration also increased. Transcriptomic analysis revealed that genes related to glycolysis and tricarboxylic acid cycle play crucial roles in regulating the adaptation of mud crab to hypoxia stress.