Journal
COLLOIDS AND SURFACES B-BIOINTERFACES
Volume 204, Issue -, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.colsurfb.2021.111783
Keywords
Nanozyme; Alloy nanoparticles; Peroxidase-like activity; Cascade enzyme reaction; Glucose determination
Funding
- Basic Science Research Program through the National Research Foundation (NRF) of Korea [2018R1A6A1A03024231, 2021R1A2C1003566]
- National Research Foundation of Korea [2021R1A2C1003566, 5199990214171] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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Nanozymes, particularly Pt-Ru nanostructures stabilized by PVP, exhibit intense peroxidase-like activity and are promising for glucose detection. The colorimetric and fluorometric assays showed excellent sensitivity, with the fluorometric assay having a lower detection limit. Furthermore, the PtRu nanozymes had high stability and recovery values in human serum and artificial urine, indicating their potential applications in various fields.
Nanozymes have significant advantages over natural enzymes. The intrinsic peroxidase-like activity of Pt-based nanomaterials can be enhanced by alloying with other transition metals, such as Ru, that have great catalytic activity. In this study, we used polyvinylpyrrolidone (PVP) to synthesize well-dispersed and homogeneous nanostructures. PVP-stabilized Pt-Ru nanozymes (PVP/PtRu NZs) were synthesized and characterized. The PVP/ PtRu NZs had an average size of 3.54 +/- 0.84 nm and exhibited an intense peroxidase-like activity. The PVP/PtRu NZs were used as peroxidase mimics for colorimetric and fluorometric glucose determination by the glucose oxidase and PVP/PtRu NZs cascade reaction. In the colorimetric assay, the linearly detectable range was 0.25-3.0 mM, with an R2 and limit of detection (LOD) of 0.988 and 138 mu M, respectively. In the fluorometric assay, a linear relationship was found when the glucose concentration was between 5.0 and 300 mu M (R2 = 0.997), with an LOD of 1.11 mu M. Compared to the colorimetric assay, the fluorometric assay had greater sensitivity and a lower detection limit for the determination of glucose. Moreover, the PVP/PtRu NZs had high storage stability over a month and great recovery values in human serum and artificial urine, with a range of 94-106 %. From these results, PVP/PtRu NZs are expected to be used as promising peroxidase mimics in various fields such as biosensing, pharmaceutical processing, and the food industry.
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