4.7 Article

Monitoring the SARS-CoV-2 pandemic: screening algorithm with single nucleotide polymorphism detection for the rapid identification of established and emerging variants

Related references

Note: Only part of the references are listed.
Article Microbiology

Identification of SARS-CoV-2 spike mutations that attenuate monoclonal and serum antibody neutralization

Zhuoming Liu et al.

Summary: The study found that antibodies targeting the SARS-CoV-2 spike protein have escape mutations, different monoclonal antibodies have unique resistance profiles, some mutants are resistant to multiple antibodies while some variants can escape neutralization by convalescent sera. Comparing antibody-mediated mutations with circulating SARS-CoV-2 sequences revealed substitutions that may weaken neutralizing immune responses in some individuals, warranting further investigation.

CELL HOST & MICROBE (2021)

Article Infectious Diseases

Novel SARS-CoV-2 variants: the pandemics within the pandemic

Erik Boehm et al.

Summary: Many new variants of SARS-CoV-2 have been identified as variants of concern/interest due to increased transmissibility, severity, immune escape, and reduced vaccine efficacy. These variants often share similar mutations, such as N501Y and E484K, which lead to partial immune escape, decreased vaccine efficacy, and potentially increased severity.

CLINICAL MICROBIOLOGY AND INFECTION (2021)

Letter Medicine, General & Internal

S-Gene Target Failure as a Marker of Variant B.1.1.7 Among SARS-CoV-2 Isolates in the Greater Toronto Area, December 2020 to March 2021

Kevin A. Brown et al.

JAMA-JOURNAL OF THE AMERICAN MEDICAL ASSOCIATION (2021)

Article Biochemistry & Molecular Biology

Multiplex qPCR discriminates variants of concern to enhance global surveillance of SARS-CoV-2

Chantal B. F. Vogels et al.

Summary: With the emergence of SARS-CoV-2 variants with increased transmissibility and immune escape, targeted surveillance is urgently needed. The B.1.1.7 variant can be detected by a specific deletion in the spike gene, but other variants like B.1.351 and P.1 cannot. An open-source PCR assay targeting a deletion in the ORF1a gene has been designed and validated to enhance surveillance for these variants.

PLOS BIOLOGY (2021)

Review Microbiology

SARS-CoV-2 variants, spike mutations and immune escape

William T. Harvey et al.

Summary: The evolution of SARS-CoV-2 has been characterized by the emergence of mutations and variants that impact virus characteristics. Manufacturers are preparing for possible updates to vaccines in response to changes in the virus population, and it is crucial to monitor genetic and antigenic changes alongside experiments to understand the impacts of mutations.

NATURE REVIEWS MICROBIOLOGY (2021)

Article Immunology

Rapid Detection of SARS-CoV-2 Variants of Concern, Including B.1.1.28/P.1, British Columbia, Canada

Nancy Matic et al.

Summary: In Vancouver, British Columbia, Canada, a real-time reverse transcription PCR-based algorithm was used to screen all severe acute respiratory syndrome coronavirus 2-positive samples and identify variants of concern. A total of 77 samples with variants were rapidly identified, with majority being B.1.1.7 and 8.1.351 variants.

EMERGING INFECTIOUS DISEASES (2021)

Article Multidisciplinary Sciences

Illumina sequencing of clinical samples for virus detection in a public health laboratory

Bixing Huang et al.

SCIENTIFIC REPORTS (2019)

Article Multidisciplinary Sciences

Data, disease and diplomacy: GISAID's innovative contribution to global health

Stefan Elbe et al.

GLOBAL CHALLENGES (2017)