What do we need to obtain high quality circulating tumor DNA (ctDNA) for routine diagnostic test in oncology?-Considerations on pre-analytical aspects by the IFCC workgroup cfDNA

The analysis of circulating cell free DNA is an important tool for the analysis of tumor resistance, tumor heterogeneity, detection of minimal residual disease and detection of allograft rejection in kidney or heart transplant patients. The proper use of this technique is important, and starts with considering pre-analytic aspects. The current paper addresses some important technical considerations to ensure the proper and harmonized use of cfDNA techniques.

Automated summary

The analysis of circulating cell free DNA is crucial for studying tumor resistance, heterogeneity, detecting minimal residual disease, and allograft rejection in transplant patients. Proper use of this technique begins with pre-analytic considerations. This paper discusses important technical considerations to ensure the appropriate and coordinated use of cfDNA techniques.