Journal
CHROMATOGRAPHIA
Volume 84, Issue 9, Pages 861-868Publisher
SPRINGER HEIDELBERG
DOI: 10.1007/s10337-021-04075-2
Keywords
Capillary electrophoresis; Indirect laser-induced fluorescence detection; Neomycin; Fish
Funding
- National Natural Science Foundation of China (NSFC) [21005021]
- Natural Science Foundation of Guangdong Province [2016A030313740, 2021A1515011410]
- Guangdong Provincial Science and Technology Project [2016B030303002]
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A capillary electrophoresis-indirect laser-induced fluorescence detection method was established for neomycin detection in fish. With optimal conditions, the limit of detection of neomycin was 5.0 ng/g, the recovery of fish samples is between 95.2 and 99.7%, and the relative standard deviation is between 2.7 and 3.6%. The study also investigated the effects of background buffer, pH, and separation voltage on the separation and analysis process.
A capillary electrophoresis-indirect laser-induced fluorescence detection method was established for neomycin detection in fish. With rhodamine 6G as the background fluorescent substance, neomycin with weak ultraviolet absorption can be detected effectively. The types and concentrations of background buffer, pH, and separation voltage which affected the separation and analysis were all studied. With the optimal conditions, the limit of detection of neomycin was 5.0 ng/g, the recovery of fish samples is between 95.2 and 99.7%, and the relative standard deviation is between 2.7 and 3.6%.
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