Selective Removal of Florfenicol from Fetal Bovine Serum by Restricted Access Media-Magnetic Molecularly Imprinted Polymers

Unique restricted access media-magnetic molecularly imprinted polymers (RAM-MMIPs) were prepared as magnetic solid-phase extraction (MSPE) material for florfenicol (FF) and are combined with HPLC-UV to extract FF from fetal bovine serum samples. Under the optimization conditions, the resulting materials had a uniform imprinted layer, acceptable adsorption amounts (22.92 mg/g), rapid binding kinetics (80 min) and satisfactory selectivity. In addition, RAM-MMIPs presented satisfactory recoveries (95.6-102.1%), relative standard deviation (RSD) (2.7-3.9%) and lower limits of detection (LOD) (10.5 mu g/L). The method was applied to the real samples. Compared with other reported methods, this method exhibits the advantages of simple, fast and, efficient operation, using a relatively small amount of the RAM-MMIPs adsorbent. The developed method can enrich FF from fetal bovine serum samples.

Automated summary

Unique restricted access media-magnetic molecularly imprinted polymers (RAM-MMIPs) were prepared and used for the extraction of florfenicol (FF) from fetal bovine serum samples. The optimized conditions resulted in materials with uniform imprinted layer, acceptable adsorption amounts, rapid binding kinetics, and satisfactory selectivity. The method showed advantages of simple, fast, and efficient operation, with relatively small amount of RAM-MMIPs adsorbent used.