4.5 Article

Conjugation of a zwitterionic polymer with dimethyl chains to lipase significantly increases the enzyme activity and stability

Journal

CHINESE JOURNAL OF CHEMICAL ENGINEERING
Volume 47, Issue -, Pages 48-53

Publisher

CHEMICAL INDUSTRY PRESS CO LTD
DOI: 10.1016/j.cjche.2021.04.023

Keywords

Lipase; Zwitterionic polymer; Enzyme-polymer conjugate; Stability; Interfacial activation

Funding

  1. National Key Research and Devel-opment Program of China [2018YFA0900702]
  2. National Natural Science Foundation of China [21621004]

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This study successfully developed a novel enzyme-polymer conjugate by covalently coupling a zwitterionic polymer with side dimethyl chains (pID) to Candida rugosa lipase (CRL). The resulting conjugates showed higher catalytic activity, thermostability, and pH tolerance compared to the native enzyme. The findings suggest that enzyme conjugation to zwitterionic polymers has promising potential for improving enzyme performance.
Enzyme-polymer conjugates are complex molecules with great practical significance. This work was designed to develop a novel enzyme-polymer conjugate by covalently coupling a zwitterionic polymer with side dimethyl chains (pID) to Candida rugosa lipase (CRL) via the reaction between the anhydrides of polymer chains with the amino groups of the enzyme. The resulting two CRL-pID conjugates with different pID grafting densities were investigated in term of the catalytic activity, stability and structural changes. In comparison with native CRL, both the CRL conjugates displayed 2.2 times higher activity than the native enzyme, and showed an increase in the maximum reaction rate (V-max) and a decrease in the Michaelis constant (K-m), thus resulting in about three-fold increases in the catalytic efficiency (k(ca)(t)/K-m). These are mainly attributed to the activation of lipase by the hydrophobic alky side chains. Moreover, the thermostability and pH tolerance of the lipase conjugates were significantly enhanced due to the stabilizing effect of the zwitterion moieties. For instance, a five-fold increase of the enzyme half-life at 50 degrees C for the high-pID conjugated CRL was observed. Spectroscopic studies reveal that the pID conjugation protected the enzyme in the changes in its microenvironment and conformation, well correlating with enhanced activity and stability of lipase conjugates. The findings indicate that enzyme conjugation to the zwitterionic polymer is promising for improving enzyme performance and deserves further development. (C) 2021 The Chemical Industry and Engineering Society of China, and Chemical Industry Press Co., Ltd. All rights reserved.

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