Journal
CHINESE CHEMICAL LETTERS
Volume 33, Issue 6, Pages 2893-2900Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.cclet.2021.09.058
Keywords
Microfluidics; Single-cell EV analysis; Single EV analysis; Extracellular vesicle; Heterogeneity
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Funding
- National Natural Science Foundation of China [21874133, 31927802]
- Youth Innovation Promotion Association CAS [2018217]
- Dalian Institute of Chemical Physics, CAS [I201908]
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Extracellular vesicles (EVs) are membrane vesicles secreted by cells, playing critical roles in intercellular communication. Most EV analysis is currently performed at the bulk level, but microfluidic platforms are emerging to enable single-cell and single-vesicle analysis for comprehensive evaluation of EVs and decoding their heterogeneity.
Extracellular vesicles (EVs) are membrane vesicles secreted by cells, playing critical roles in mediating intercellular communications for various physiological and pathological processes. Most of the EV analysis is currently performed at the bulk level, obscuring the origin of the EVs and diverse characteristics of the individual extracellular vesicle. Technologies to analyze the extracellular vesicles at the single-cell and single-vesicle levels are needed to evaluate EV comprehensively and decode the heterogeneity underlying EV secretion. Microfluidic platforms that could control and manipulate fluids at the microscale provide an efficient way to achieve the aims. Various microfluidics-based technologies are emerging to realize single-cell EV secretion analysis and single EV analysis, which would be summarized in this mini-review. (C) 2021 Published by Elsevier B.V. on behalf of Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences.
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