Chlorella vulgaris phycoremediation at low Cu+2 contents: Proteomic profiling of microalgal metabolism related to fatty acids and CO2 fixation

The aim of this work was to correlate metabolic changes with copper ions (Cu+2) bioremediation by microalgae C. vulgaris 097 CCMA-UFSCar at low Cu+2 content. The metabolic effects include proteome changes related to fatty acid biosynthesis (value-added product) and carbon fixation (climate change mitigation). Cu+2, even at low concentration, showed a significant negative impact on C. vulgaris growth. The microalgal bioremediation reached 100, 74, 38 and 26% for Cu+2 content at 0.1; 0.3; 0.6 and 0.9 mg L-1, respectively. Regarding proteomics, the numbers of proteins reduced (approximate to 37%) from 581 proteins (control) to 369 proteins (0.9 mg of Cu+2 L-1) compared to control. The microalgal CO2 fixation is strictly related to the Calvin cycle, particularly phase 1, in which ribulose bisphosphate carboxylase large chain (RuBisCO) produces two phosphoglycerate molecules from CO2 and ribulose 1,5-bisphosphate. Then, phosphoglycerate can be metabolically reduced into glucose. When compared to control, the RuBisCO was underexpressed (approximate to 50%). Similar changes in proteomic profiling of metabolism-related to fatty acid biosynthesis was observed. Nevertheless, no protein was found for the cultivation at 0.9 mg of Cu+2 L-1. Thus, the analysis of C. vulgaris proteomic data indicated that even at low concentration, Cu+2 lead to drastic metabolic changes.

Automated summary

This study aimed to investigate the correlation between metabolic changes and copper ions (Cu+2) bioremediation by microalgae C. vulgaris 097 CCMA-UFSCar at low Cu+2 content. The results showed that even at low concentrations, Cu+2 had a significant negative impact on C. vulgaris growth, leading to proteome changes related to fatty acid biosynthesis and carbon fixation. The analysis of C. vulgaris proteomic data indicated drastic metabolic changes at low concentrations of Cu+2.