Journal
CHEMICAL ENGINEERING JOURNAL
Volume 428, Issue -, Pages -Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.cej.2021.131073
Keywords
Solid-phase extraction; Chlorophylls; Carotenoids; Resin reuse; Surface-active ionic liquids; Eluents
Categories
Funding
- Portuguese Foundation for Science and Technology/MCTES [UIDB/50011/2020, UIDP/50011/2020]
- University of Aveiro [UIDB/50006/2020]
- FCT/MCT [UIDB/50006/2020]
- Fundacao para a Ciencia e a Tecnologia (FCT) [SFRH/BD/122220/2016]
- FCT, I.P. [CEE-CIND/00383/2017]
- FEDER through COMPETE 2020 [022161]
- FEDER through POCI [022161]
- FEDER through PORL [022161]
- FCT through PIDDAC [022161]
- Fundação para a Ciência e a Tecnologia [SFRH/BD/122220/2016] Funding Source: FCT
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There is a need for convenient and fast techniques to purify biomolecules, and solid-phase extraction can help separate compounds in complex extracts. This study proposed an innovative procedure for eluting chlorophyll, showing that the resin can be reused without losing efficiency.
There is a need for handy and fast techniques to purify biomolecules, increasing their stability and value, because the separation units are current bottlenecks in downstream processes. Solid-phase extraction is a technique that enables the purification of a compound by its adsorption from a liquid matrix. The AmberLiteTM HPR900 OH resin allows the separation of chlorophylls from complex extracts, however the recovery of the chlorophylls is not easy to achieve. An innovative procedure to elute the chlorophyll from AmberLiteTM HPR900 OH, based on the use of aqueous solutions of surface-active ionic liquids is proposed in this work. The operational conditions were optimized, showing that the resin can be reused for at least five cycles without losing its efficiency and the chemical structures of the pigments recovered were identified.
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